Coding

Part:BBa_K3799002:Design

Designed by: Shubhamay Das,Lakshmi Prakash   Group: iGEM21_IISER_Kolkata   (2021-09-30)


Bovine pancreatic DNase I,serum endonuclease of Bos taurus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design notes

The sequences were obtained synthetically and Codon optimized for expression in E. Coli host

Source

Bos taurus (Bovine), https://www.ncbi.nlm.nih.gov/gene/282217 Obtained from the serum secretion pancreatic cell of Bos taurus We have obtained the DNA from IDT

References

Sharma K, Pagedar Singh A. Antibiofilm Effect of DNase against Single and Mixed Species Biofilm. Foods. 2018;7(3):42. Published 2018 Mar 19. doi:10.3390/foods7030042 https://www.ncbi.nlm.nih.gov/gene/282217